Hotspot

trnS-GCU-trnR-UCU

LSC · 9036-10611 · 1576 bp

Back to Anthoshorea

Pi 0.0217

MarkerSeek score 64.00

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1576
variable_sites: 34
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	AGCAAAACGGAAAGAGAGGG	TGTCCACTAAATTGCGTCCA	50.0	45.0	58.1	57.4	1755-1850 bp	1.0	76.7
trnS-GCU-trnR-UCU_p2	GCAAAACGGAAAGAGAGGGA	TGTCCACTAAATTGCGTCCA	50.0	45.0	58.1	57.4	1754-1849 bp	1.0	76.7
trnS-GCU-trnR-UCU_p3	ACGGAAAGAGAGGGATTCGA	TGTCCACTAAATTGCGTCCA	50.0	45.0	58.2	57.4	1749-1844 bp	1.0	76.3
trnS-GCU-trnR-UCU_p4	AGCAAAACGGAAAGAGAGGG	GTGTCCACTAAATTGCGTCC	50.0	50.0	58.1	57.1	1756-1851 bp	1.0	74.0
trnS-GCU-trnR-UCU_p5	GCAAAACGGAAAGAGAGGGA	GTGTCCACTAAATTGCGTCC	50.0	50.0	58.1	57.1	1755-1850 bp	1.0	73.9