Hotspot

rps16-trnQ-UUG

LSC · 6136-7706 · 1571 bp

Back to Anthoshorea

Pi 0.0311

MarkerSeek score 67.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1571
variable_sites: 48
pi: 0.0311
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	TTTGGTCCCGGTGATCAAAG	45.0	50.0	58.1	58.1	1661-1902 bp	1.0	81.4
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	CCGGTGATCAAAGGTTCGAA	45.0	50.0	58.1	58.2	1654-1895 bp	1.0	80.6
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	AATAGACAGAATGGGGCGTG	45.0	50.0	58.1	57.7	1703-1944 bp	1.0	79.5
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GTTTTGGTCCCGGTGATCAA	45.0	50.0	58.1	58.4	1663-1904 bp	1.0	79.1
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GTCCCGGTGATCAAAGGTTC	45.0	55.0	58.1	58.5	1657-1898 bp	1.0	77.8