Hotspot

cysA

Genome · 43793-44941 · 1149 bp

Back to Anthoceros

Pi 0.00609

MarkerSeek score 62.30

Primer available yes

Label cysA

Feature Metadata

label_name: cysA
feature_type: gene
region: Genome
length_bp: 1149
variable_sites: 7
pi: 0.00609
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cysA_p1	TGAAATTCTTTATGATGGGAGA	AGCAAGAATAGCAAAGAAAGCC	31.8	40.9	52.8	57.6	1257 bp	1.0	40.4
cysA_p2	TGAAATTCTTTATGATGGGAGA	GCAAGAATAGCAAAGAAAGCCT	31.8	40.9	52.8	57.6	1256 bp	1.0	40.4
cysA_p3	TGAAATTCTTTATGATGGGAGA	TCTCCAGAAAGCAAGAATAGCA	31.8	40.9	52.8	57.4	1266 bp	1.0	40.4
cysA_p4	TGAAATTCTTTATGATGGGAGAA	AGCAAGAATAGCAAAGAAAGCC	30.4	40.9	53.7	57.6	1257 bp	1.0	40.4
cysA_p5	TGAAATTCTTTATGATGGGAGAA	GCAAGAATAGCAAAGAAAGCCT	30.4	40.9	53.7	57.6	1256 bp	1.0	40.4