Hotspot

atpI

Genome · 15158-15904 · 747 bp

Back to Anthoceros

Pi 0.00402

MarkerSeek score 61.10

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: Genome
length_bp: 747
variable_sites: 3
pi: 0.00402
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	TCACAATCAATTTTTCAAAGAGGT	TGCGAAAAATGAAAACTTACA	29.2	28.6	55.7	52.9	828 bp	1.0	40.3
atpI_p2	TCACAATCAATTTTTCAAAGAGGT	TTGCGAAAAATGAAAACTTACA	29.2	27.3	55.7	53.7	829 bp	1.0	40.3
atpI_p3	TTCACAATCAATTTTTCAAAGAGGT	TGCGAAAAATGAAAACTTACA	28.0	28.6	56.4	52.9	829 bp	1.0	40.3
atpI_p4	TCACAATCAATTTTTCAAAGAGGT	TTTGCGAAAAATGAAAACTTACA	29.2	26.1	55.7	54.5	830 bp	1.0	40.3
atpI_p5	TCACAATCAATTTTTCAAAGAGGT	TGCGAAAAATGAAAACTTACAT	29.2	27.3	55.7	53.5	828 bp	1.0	40.3