Hotspot

ORF288

Genome · 126451-127317 · 867 bp

Back to Anthoceros

Pi 0.00461

MarkerSeek score 61.40

Primer available yes

Label ORF288

Feature Metadata

label_name: ORF288
feature_type: gene
region: Genome
length_bp: 867
variable_sites: 4
pi: 0.00461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF288_p1	GGCTAATCCGAGATTTTGAGA	ACTATCATTTCCTTCTTTTCAGA	42.9	30.4	55.5	53.4	1002-1003 bp	1.0	40.4
ORF288_p2	GGCTAATCCGAGATTTTGAGA	AACTATCATTTCCTTCTTTTCAGA	42.9	29.2	55.5	54.2	1003-1004 bp	1.0	40.4
ORF288_p3	GGCTAATCCGAGATTTTGAGAA	ACTATCATTTCCTTCTTTTCAGA	40.9	30.4	56.2	53.4	1002-1003 bp	1.0	40.4
ORF288_p4	GGCTAATCCGAGATTTTGAGA	ACTATCATTTCCTTCTTTTCAGAT	42.9	29.2	55.5	54.0	1002-1003 bp	1.0	40.4
ORF288_p5	GGCTAATCCGAGATTTTGAGAA	AACTATCATTTCCTTCTTTTCAGA	40.9	29.2	56.2	54.2	1003-1004 bp	1.0	40.4