Hotspot

chlL-trnN

Genome · 144919-145781 · 863 bp

Back to Anthoceros

Pi 0.00812

MarkerSeek score 63.50

Primer available yes

Label chlL-trnN

Feature Metadata

label_name: chlL-trnN
feature_type: igs
region: Genome
length_bp: 863
variable_sites: 7
pi: 0.00812
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL-trnN_p1	ACCAATTCCACCTTTCCCAT	AACAAAGCAAGCCCTTACCA	45.0	45.0	57.0	57.9	1001-1046 bp	1.0	71.8
chlL-trnN_p2	ACCAATTCCACCTTTCCCAT	ACAAAGCAAGCCCTTACCAA	45.0	45.0	57.0	57.9	1000-1045 bp	1.0	71.8
chlL-trnN_p3	ACCAATTCCACCTTTCCCAT	CCTCAGGTGGGATTTGAACC	45.0	55.0	57.0	58.2	969-1014 bp	1.0	71.2
chlL-trnN_p4	CCACCTTTCCCATAAACTGCT	AACAAAGCAAGCCCTTACCA	47.6	45.0	58.2	57.9	994-1039 bp	1.0	70.4
chlL-trnN_p5	CCACCTTTCCCATAAACTGCT	ACAAAGCAAGCCCTTACCAA	47.6	45.0	58.2	57.9	993-1038 bp	1.0	70.4