Hotspot

petG-trnW

Genome · 84383-84610 · 228 bp

Back to Anthoceros

Pi 0.0219

MarkerSeek score 65.10

Primer available yes

Label petG-trnW

Feature Metadata

label_name: petG-trnW
feature_type: igs
region: Genome
length_bp: 228
variable_sites: 5
pi: 0.0219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW_p1	TGGTTGAAGCTTTGCTGTCT	TCGGTAGAACGTAGGTCTCC	45.0	55.0	57.9	58.0	399 bp	1.0	80.3
petG-trnW_p2	TGGTTGAAGCTTTGCTGTCT	CGGTAGAACGTAGGTCTCCA	45.0	55.0	57.9	58.3	398 bp	1.0	78.5
petG-trnW_p3	TGAAGCTTTGCTGTCTGGAA	TCGGTAGAACGTAGGTCTCC	45.0	55.0	57.7	58.0	395 bp	1.0	78.0
petG-trnW_p4	TTGAAGCTTTGCTGTCTGGA	TCGGTAGAACGTAGGTCTCC	45.0	55.0	57.7	58.0	396 bp	1.0	78.0
petG-trnW_p5	TGGTTGAAGCTTTGCTGTCT	CGCTCTTAGTTCAGTTCGGT	45.0	50.0	57.9	57.7	414 bp	1.0	77.7