Hotspot

atpB-rbcL

Genome · 72086-73270 · 1185 bp

Back to Anthoceros

Pi 0.00613

MarkerSeek score 65.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: Genome
length_bp: 1185
variable_sites: 7
pi: 0.00613
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TATGCCCCACATTCTTGGAG	TTGGTCTCGTAATCAGGGGT	50.0	50.0	57.3	58.1	1298-1341 bp	1.0	76.8
atpB-rbcL_p2	TATGCCCCACATTCTTGGAG	GTCTCCGTTTGTGGTGACAT	50.0	50.0	57.3	58.1	1223-1266 bp	1.0	76.4
atpB-rbcL_p3	ATGCCCCACATTCTTGGAGA	TTGGTCTCGTAATCAGGGGT	50.0	50.0	59.0	58.1	1297-1340 bp	1.0	74.7
atpB-rbcL_p4	TGCCCCACATTCTTGGAGAT	TTGGTCTCGTAATCAGGGGT	50.0	50.0	59.0	58.1	1296-1339 bp	1.0	74.7
atpB-rbcL_p5	GCCCCACATTCTTGGAGATA	TTGGTCTCGTAATCAGGGGT	50.0	50.0	57.0	58.1	1295-1338 bp	1.0	74.4