Hotspot

trnH-ycf2

Genome · 33375-33852 · 478 bp

Back to Anthoceros

Pi 0.00637

MarkerSeek score 61.30

Primer available yes

Label trnH-ycf2

Feature Metadata

label_name: trnH-ycf2
feature_type: igs
region: Genome
length_bp: 478
variable_sites: 3
pi: 0.00637
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-ycf2_p1	CATTATGCGCGGGTTCAATC	TCCTGTATGAAAATTTGTTTTCT	50.0	26.1	58.0	52.8	547-551 bp	1.0	41.5
trnH-ycf2_p2	TGGATTACGGCAGTGGATTG	TCCTGTATGAAAATTTGTTTTCT	50.0	26.1	58.0	52.8	573-577 bp	1.0	41.4
trnH-ycf2_p3	GGATTACGGCAGTGGATTGT	TCCTGTATGAAAATTTGTTTTCT	50.0	26.1	58.0	52.8	572-576 bp	1.0	41.4
trnH-ycf2_p4	GTGGATTACGGCAGTGGATT	TCCTGTATGAAAATTTGTTTTCT	50.0	26.1	58.0	52.8	574-578 bp	1.0	41.4
trnH-ycf2_p5	GATATTGAAAGGGCGGACGT	TCCTGTATGAAAATTTGTTTTCT	50.0	26.1	58.1	52.8	600-604 bp	1.0	41.3