Hotspot

ORF51-trnK

Genome · 28025-28902 · 878 bp

Back to Anthoceros

Pi 0.0137

MarkerSeek score 53.10

Primer available yes

Label ORF51-trnK

Feature Metadata

label_name: ORF51-trnK
feature_type: igs
region: Genome
length_bp: 878
variable_sites: 12
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF51-trnK_p1	GCTCAATGTCGGAGAGAAGG	GCACTTGAAAGCCGAGTACT	55.0	50.0	58.1	58.2	1270-1271 bp	1.0	78.8
ORF51-trnK_p2	CAATGTCGGAGAGAAGGACG	GCACTTGAAAGCCGAGTACT	55.0	50.0	58.1	58.2	1267-1268 bp	1.0	78.7
ORF51-trnK_p3	CTAGAGAAGGGGCTCAACCT	GCACTTGAAAGCCGAGTACT	55.0	50.0	58.1	58.2	1170-1171 bp	1.0	78.4
ORF51-trnK_p4	AATGTCGGAGAGAAGGACGA	GCACTTGAAAGCCGAGTACT	50.0	50.0	58.2	58.2	1266-1267 bp	1.0	78.1
ORF51-trnK_p5	GGGGCTCAACCTACAGAAAC	GCACTTGAAAGCCGAGTACT	55.0	50.0	58.2	58.2	1162-1163 bp	1.0	78.0