Hotspot

ycf1

SSC · 124942-130161 · 5220 bp

Back to Anthericum

Pi 0.0113

MarkerSeek score 55.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5220
variable_sites: 59
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATTTTGGCGACCCATCTTGT	ACCCCGAATTGCAAACTTCT	45.0	45.0	58.1	58.0	670 bp	1.0	80.8
ycf1_p2	ATTTTGGCGACCCATCTTGT	ACACGTGCATTCTCCACTTT	45.0	45.0	58.1	58.0	780 bp	1.0	80.7
ycf1_p3	ATTTTGGCGACCCATCTTGT	GCACGAGGATTGGAAGGAAT	45.0	50.0	58.1	58.0	423 bp	1.0	80.5
ycf1_p4	GCTTGTTTCGGCGTTGTAAA	ACCCCGAATTGCAAACTTCT	45.0	45.0	57.9	58.0	1181 bp	1.0	80.2
ycf1_p5	GCTTGTTTCGGCGTTGTAAA	ACACGTGCATTCTCCACTTT	45.0	45.0	57.9	58.0	1291 bp	1.0	80.1