Hotspot

rpl22

LSC · 84308-84706 · 399 bp

Back to Anthericum

Pi 0.00752

MarkerSeek score 44.00

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 399
variable_sites: 3
pi: 0.00752
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TCGAAGCCTGGGGATATTCT	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	969-1018 bp	1.0	84.4
rpl22_p2	TCGAAGCCTGGGGATATTCT	TTATCCTGCGCTTGGAAGAC	50.0	50.0	57.9	58.0	968-1017 bp	1.0	84.4
rpl22_p3	TCGAAGCCTGGGGATATTCT	CATTCATTGGCGGGGGATAA	50.0	50.0	57.9	58.0	650-654 bp	1.0	80.5
rpl22_p4	TCCAATTCCTTCGAAGCCTG	CATTCATTGGCGGGGGATAA	50.0	50.0	57.9	58.0	660-664 bp	1.0	80.4
rpl22_p5	GTCTTCGGTTCACAGAACGA	CATTCATTGGCGGGGGATAA	50.0	50.0	57.9	58.0	811-815 bp	1.0	80.1