Hotspot

rpl16

LSC · 82087-83444 · 1358 bp

Back to Anthericum

Pi 0.0118

MarkerSeek score 57.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1358
variable_sites: 16
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCATCCTCAATACCCCTTTTGT	GTTCTGCCCGATGATAGAACA	40.9	47.6	57.1	57.8	1514-1519 bp	1.0	47.8
rpl16_p2	TCATCCTCAATACCCCTTTTGTT	GTTCTGCCCGATGATAGAACA	39.1	47.6	57.7	57.8	1514-1519 bp	1.0	45.3
rpl16_p3	TTCATCCTCAATACCCCTTTTGT	GTTCTGCCCGATGATAGAACA	39.1	47.6	57.7	57.8	1515-1520 bp	1.0	45.3
rpl16_p4	TCATCCTCAATACCCCTTTTGT	TCTGCCCGATGATAGAACAA	40.9	45.0	57.1	56.3	1512-1517 bp	1.0	44.0
rpl16_p5	TCATCCTCAATACCCCTTTTGT	TTCTGCCCGATGATAGAACA	40.9	45.0	57.1	56.3	1513-1518 bp	1.0	44.0