Hotspot

ycf1-ndhF

SSC · 111120-111126 · 7 bp

Back to Anthericum

Pi 0.143

MarkerSeek score 73.00

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 7
variable_sites: 1
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	TACCAAGCTCAACGTTAGCC	TATGGGAGGTGGACGAGTTT	50.0	50.0	57.9	58.1	619-625 bp	1.0	79.4
ycf1-ndhF_p2	CAAGCTCAACGTTAGCCAGA	TATGGGAGGTGGACGAGTTT	50.0	50.0	58.2	58.1	616-622 bp	1.0	78.5
ycf1-ndhF_p3	TACCAAGCTCAACGTTAGCC	ATATGGGAGGTGGACGAGTT	50.0	50.0	57.9	57.8	620-626 bp	1.0	78.4
ycf1-ndhF_p4	ATCCGCCGAAGAAGACAAAG	TATGGGAGGTGGACGAGTTT	50.0	50.0	58.3	58.1	249 bp	0.5	57.4
ycf1-ndhF_p5	CCGCCGAAGAAGACAAAGAT	TATGGGAGGTGGACGAGTTT	50.0	50.0	58.3	58.1	247 bp	0.5	57.4