Hotspot

psbT-psbN

LSC · 75142-75210 · 69 bp

Back to Anthericum

Pi 0.0435

MarkerSeek score 75.90

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 69
variable_sites: 3
pi: 0.0435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTTTCGAGAACCGCCTAAGG	ACCCTAGTCGCCATCTTCAT	50.0	50.0	57.9	58.2	226 bp	1.0	78.7
psbT-psbN_p2	TTTTCGAGAACCGCCTAAGG	GGGTACGCCTTATATACCGC	50.0	55.0	57.9	57.7	181 bp	1.0	78.3
psbT-psbN_p3	TTTTCGAGAACCGCCTAAGG	GATCCATTCGAGGAACACGG	50.0	55.0	57.9	58.4	133 bp	1.0	77.7
psbT-psbN_p4	TTTTCGAGAACCGCCTAAGG	AGATCCATTCGAGGAACACG	50.0	50.0	57.9	57.4	134 bp	1.0	76.4
psbT-psbN_p5	TTTTCGAGAACCGCCTAAGG	GGCAACCCTCTCAACAACTA	50.0	50.0	57.9	57.4	156 bp	1.0	76.3