Hotspot

atpB-rbcL

LSC · 55325-56132 · 808 bp

Back to Anthericum

Pi 0.0175

MarkerSeek score 69.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 14
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CGGGGGAAAAACAACATCCA	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.7	56.6	940-945 bp	1.0	65.6
atpB-rbcL_p2	TCTTCAAGTGTGGAAACCGC	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.7	56.6	879-884 bp	1.0	65.6
atpB-rbcL_p3	CGCAGGACCAGAAGTAGTAG	AGTCTCTGTTTGTGGTGACA	55.0	45.0	57.1	56.6	862-867 bp	1.0	64.3
atpB-rbcL_p4	CTTCAAGTGTGGAAACCGCA	AGTCTCTGTTTGTGGTGACA	50.0	45.0	59.0	56.6	878-883 bp	1.0	63.3
atpB-rbcL_p5	TTCAAGTGTGGAAACCGCAG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	59.0	56.6	877-882 bp	1.0	63.3