Hotspot

trnS-GCU-trnS-CGA

LSC · 8263-9364 · 1102 bp

Back to Anthericum

Pi 0.0164

MarkerSeek score 58.50

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 1102
variable_sites: 18
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	TGATCAGAGAGAGCGGAGAG	CGAACGAATCACACTTTTACCA	55.0	40.9	58.0	57.3	1240-1320 bp	1.0	59.4
trnS-GCU-trnS-CGA_p2	AAGTGATCAGAGAGAGCGGA	CGAACGAATCACACTTTTACCA	50.0	40.9	57.9	57.3	1243-1323 bp	1.0	58.5
trnS-GCU-trnS-CGA_p3	GATCAGAGAGAGCGGAGAGA	CGAACGAATCACACTTTTACCA	55.0	40.9	57.7	57.3	1239-1319 bp	1.0	57.6
trnS-GCU-trnS-CGA_p4	AATCTGCCGCTTTAGTCCAC	CGAACGAATCACACTTTTACCA	50.0	40.9	58.3	57.3	1173-1253 bp	1.0	57.6
trnS-GCU-trnS-CGA_p5	ATCAGAGAGAGCGGAGAGAG	CGAACGAATCACACTTTTACCA	55.0	40.9	57.7	57.3	1238-1318 bp	1.0	57.5