Hotspot

rps16-trnQ-UUG

LSC · 5813-6944 · 1132 bp

Back to Anthericum

Pi 0.016
MarkerSeek score 58.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1132
variable_sites
18
pi
0.016
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1205-1220 bp 1.0 79.7
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1216-1231 bp 1.0 79.2
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1209-1224 bp 1.0 75.0
rps16-trnQ-UUG_p4 ATCATGTCCTTCAAGTCGCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.2 58.1 1217-1232 bp 1.0 74.7
rps16-trnQ-UUG_p5 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1197-1212 bp 1.0 74.6