Hotspot

rpl16

LSC · 79730-81371 · 1642 bp

Back to Anoectochilus

Pi 0.00563

MarkerSeek score 51.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1642
variable_sites: 34
pi: 0.00563
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGAGCAATCGCACCAAAAAT	GCAGGGTTCCCTTACAAACA	45.0	50.0	58.0	58.0	2344-2364 bp	1.0	82.4
rpl16_p2	CGAGCAATCGCACCAAAAAT	CAAGTACAAATTGCAGGGCG	45.0	50.0	58.0	58.0	2408-2428 bp	1.0	82.3
rpl16_p3	CGCACCAAAAATCCGAGTTC	GCAGGGTTCCCTTACAAACA	50.0	50.0	58.0	58.0	2336-2356 bp	1.0	82.3
rpl16_p4	CGCACCAAAAATCCGAGTTC	CAAGTACAAATTGCAGGGCG	50.0	50.0	58.0	58.0	2400-2420 bp	1.0	82.1
rpl16_p5	TCTCAATTCCCGAGCAATCG	GCAGGGTTCCCTTACAAACA	50.0	50.0	58.1	58.0	2354-2374 bp	1.0	82.0