Hotspot

rpl16-rps3

LSC · 81372-81549 · 178 bp

Back to Anoectochilus

Pi 0.0283

MarkerSeek score 62.20

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 178
variable_sites: 13
pi: 0.0283
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	TATAGTGGGGTCAGCCTGTT	GCAGGGTTCCCTTACAAACA	50.0	50.0	57.7	58.0	360-362 bp	0.9	77.1
rpl16-rps3_p2	TTATAGTGGGGTCAGCCTGT	GCAGGGTTCCCTTACAAACA	50.0	50.0	57.7	58.0	361-363 bp	0.9	77.1
rpl16-rps3_p3	AACGAGTCACACACTAAGCA	GCAGGGTTCCCTTACAAACA	45.0	50.0	57.1	58.0	304-306 bp	1.0	76.8
rpl16-rps3_p4	ATAGTGGGGTCAGCCTGTTT	GCAGGGTTCCCTTACAAACA	50.0	50.0	58.6	58.0	359-361 bp	0.9	74.2
rpl16-rps3_p5	TAGTGGGGTCAGCCTGTTTT	GCAGGGTTCCCTTACAAACA	50.0	50.0	58.9	58.0	358-360 bp	0.9	72.4