Hotspot

petA-psbJ

LSC · 61616-62582 · 967 bp

Back to Anoectochilus

Pi 0.00938

MarkerSeek score 65.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 967
variable_sites: 27
pi: 0.00938
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	TATGGCTCCTATTCCGGGTT	50.0	50.0	58.0	57.9	1069-1088 bp	1.0	83.0
petA-psbJ_p2	GGCATCTGTGATTTTGGCAC	TTATGGCTCCTATTCCGGGT	50.0	50.0	58.0	57.9	1070-1089 bp	1.0	83.0
petA-psbJ_p3	GAGTCCAAGGCCTTCTGTTC	TATGGCTCCTATTCCGGGTT	55.0	50.0	58.2	57.9	1094-1113 bp	1.0	81.4
petA-psbJ_p4	GAGTCCAAGGCCTTCTGTTC	TTATGGCTCCTATTCCGGGT	55.0	50.0	58.2	57.9	1095-1114 bp	1.0	81.4
petA-psbJ_p5	GTCCAAGGCCTTCTGTTCTT	TATGGCTCCTATTCCGGGTT	50.0	50.0	57.7	57.9	1092-1111 bp	1.0	80.7