Hotspot

ycf4-cemA

LSC · 58983-59707 · 725 bp

Back to Anoectochilus

Pi 0.0046

MarkerSeek score 57.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 14
pi: 0.0046
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AAAAAGCTGCTGAATTGGCC	AAGATAAGGGAGGGAAGCCA	45.0	50.0	57.8	57.7	787-817 bp	1.0	80.6
ycf4-cemA_p2	AAAAAGCTGCTGAATTGGCC	AGATAAGGGAGGGAAGCCAA	45.0	50.0	57.8	57.7	786-816 bp	1.0	80.6
ycf4-cemA_p3	AAAGCTGCTGAATTGGCCTA	AAGATAAGGGAGGGAAGCCA	45.0	50.0	57.8	57.7	785-815 bp	1.0	80.3
ycf4-cemA_p4	AAAGCTGCTGAATTGGCCTA	AGATAAGGGAGGGAAGCCAA	45.0	50.0	57.8	57.7	784-814 bp	1.0	80.3
ycf4-cemA_p5	AGCTGCTGAATTGGCCTATT	AGATAAGGGAGGGAAGCCAA	45.0	50.0	57.6	57.7	782-812 bp	1.0	78.5