Hotspot

accD-psaI

LSC · 57347-57951 · 605 bp

Back to Anoectochilus

Pi 0.00999

MarkerSeek score 51.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 605
variable_sites: 21
pi: 0.00999
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCTACAACTGGCGGAGTTAC	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.0	57.9	955-983 bp	1.0	83.4
accD-psaI_p2	CACGGTTTCCTTCCCTTGAA	AATAGACCCGCGATCCCATA	50.0	50.0	58.0	58.1	1351-1382 bp	1.0	82.8
accD-psaI_p3	CTACATTGCGTTTACGGGGA	AATAGACCCGCGATCCCATA	50.0	50.0	58.0	58.1	1511-1542 bp	1.0	82.7
accD-psaI_p4	CCTACAACTGGCGGAGTTAC	AATAGACCCGCGATCCCATA	55.0	50.0	58.0	58.1	1579-1610 bp	1.0	82.6
accD-psaI_p5	TTGCGTTTACGGGGAAAAGA	AATAGACCCGCGATCCCATA	45.0	50.0	58.0	58.1	1506-1537 bp	1.0	82.5