Hotspot

trnT-GGU-psbD

LSC · 30842-31569 · 728 bp

Back to Anoectochilus

Pi 0.00589
MarkerSeek score 63.40
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
728
variable_sites
15
pi
0.00589
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 883-891 bp 1.0 80.9
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 886-894 bp 1.0 80.7
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 873-881 bp 1.0 79.9
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG CCCTCCGTAACCAATCATCC 55.0 55.0 58.1 57.7 857-865 bp 1.0 79.9
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 876-884 bp 1.0 79.7