Hotspot

atpH-atpI

LSC · 13016-13562 · 547 bp

Back to Anoectochilus

Pi 0.00752

MarkerSeek score 62.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 15
pi: 0.00752
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AGCCAATCCAGCAGCAATAA	AACTTTGGCTGCGGCTTATA	45.0	45.0	57.9	57.9	642-653 bp	1.0	80.8
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.8	57.9	641-652 bp	1.0	80.3
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	AACTTTGGCTGCGGCTTATA	50.0	45.0	58.3	57.9	636-647 bp	1.0	79.9
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	GGCTGCGGCTTATATAGGTG	45.0	55.0	57.9	58.3	636-647 bp	1.0	79.6
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	GGCTGCGGCTTATATAGGTG	50.0	55.0	57.8	58.3	635-646 bp	1.0	79.1