Hotspot

psbK-psbI

LSC · 6970-7430 · 461 bp

Back to Anoectochilus

Pi 0.0146

MarkerSeek score 59.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 461
variable_sites: 18
pi: 0.0146
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGCCCAGGATTAC	50.0	50.0	57.9	57.6	591-606 bp	1.0	81.2
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	588-603 bp	1.0	79.2
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	589-604 bp	1.0	79.2
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	590-605 bp	1.0	79.2
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	GGATTACGTCCCGGATCATT	50.0	50.0	57.9	57.2	578-593 bp	1.0	77.8