Hotspot

accD

LSC · 58478-60904 · 2427 bp

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Pi 0.0664
MarkerSeek score 75.60
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
2427
variable_sites
233
pi
0.0664
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.76
markerseek_score
75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 CCATTGCAATTGCCGGAAAT GAAAACGGGGAGTGATCACA 45.0 50.0 58.0 57.8 2239-2790 bp 1.0 90.4
accD_p2 AAGAAGCCATTGCAATTGCC GAAAACGGGGAGTGATCACA 45.0 50.0 57.9 57.8 2245-2796 bp 1.0 89.7
accD_p3 CCATTGCAATTGCCGGAAAT ACGACTCCATCTACGACAGA 45.0 50.0 58.0 57.6 2276-2827 bp 1.0 88.6
accD_p4 CCATTGCAATTGCCGGAAAT AAACGGGGAGTGATCACATC 45.0 50.0 58.0 57.6 2237-2788 bp 1.0 88.6
accD_p5 TATAGGGACCCGCATGCATA GAAAACGGGGAGTGATCACA 50.0 50.0 58.1 57.8 2853-2984 bp 0.5 67.6