Hotspot

trnN-GUU-ycf1

IRb · 117147-117705 · 559 bp

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Pi 0.0869
MarkerSeek score 83.90
Primer available yes
Label trnN-GUU-ycf1

Feature Metadata

label_name
trnN-GUU-ycf1
feature_type
igs
region
IRb
length_bp
559
variable_sites
87
pi
0.0869
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ycf1_p1 AAGCAAAAATGCGTTCCTCG GGCTAACGCTGAACTTGGTA 45.0 50.0 57.9 57.9 1425-2065 bp 1.0 86.4
trnN-GUU-ycf1_p2 CTTTTCCTCTGGGGGTAGGA GGCTAACGCTGAACTTGGTA 55.0 50.0 58.0 57.9 1670 bp 0.25 49.0
trnN-GUU-ycf1_p3 TCCTTTTCCTCTGGGGGTAG GGCTAACGCTGAACTTGGTA 55.0 50.0 58.0 57.9 1672 bp 0.25 49.0
trnN-GUU-ycf1_p4 CTTTTCCTCTGGGGGTAGGA AGGAAGAGATAAGAGGGCCC 55.0 55.0 58.0 57.9 1345 bp 0.25 48.8
trnN-GUU-ycf1_p5 CTTTTCCTCTGGGGGTAGGA GGAAGAGATAAGAGGGCCCT 55.0 55.0 58.0 57.9 1344 bp 0.25 48.8