Hotspot

trnL-CAA-ycf2

IRb · 93051-94097 · 1047 bp

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Pi 0.298
MarkerSeek score 78.40
Primer available yes
Label trnL-CAA-ycf2

Feature Metadata

label_name
trnL-CAA-ycf2
feature_type
igs
region
IRb
length_bp
1047
variable_sites
480
pi
0.298
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.82
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ycf2_p1 AAATGGTAGACACGCGAGAC TCGTTGATTTCTCGCCAGAG 50.0 50.0 58.0 58.0 1358-1359 bp 1.0 91.9
trnL-CAA-ycf2_p2 TGAAATGGTAGACACGCGAG TCGTTGATTTCTCGCCAGAG 50.0 50.0 58.0 58.0 1360-1361 bp 1.0 91.9
trnL-CAA-ycf2_p3 AAATGGTAGACACGCGAGAC CGTCGAGAACTCTTTGCAGA 50.0 50.0 58.0 57.9 1679-1710 bp 1.0 91.5
trnL-CAA-ycf2_p4 TGAAATGGTAGACACGCGAG CGTCGAGAACTCTTTGCAGA 50.0 50.0 58.0 57.9 1681-1712 bp 1.0 91.5
trnL-CAA-ycf2_p5 AAATGGTAGACACGCGAGAC CTGCGCTCCTTTTCTTTTGG 50.0 50.0 58.0 57.9 1831-1862 bp 1.0 91.5