Hotspot

psaC

SSC · 117367-117612 · 246 bp

Pi 0

MarkerSeek score 20.00

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 20.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TTTGCCCACTGTACATTGCT	TTTGGCCCTAGCTCCTAACT	45.0	50.0	58.0	58.0	877 bp	1.0	80.9
psaC_p2	AATCTCGAGTAAGAGGCCGA	TTTGGCCCTAGCTCCTAACT	50.0	50.0	57.9	58.0	832 bp	1.0	80.6
psaC_p3	AGCACGGATTTTGTCGGTAA	TTTGGCCCTAGCTCCTAACT	45.0	50.0	57.8	58.0	443 bp	1.0	79.4
psaC_p4	TTTGCCCACTGTACATTGCT	GCTATTGGACCCGCTATTGT	45.0	50.0	58.0	57.7	1068 bp	1.0	79.0
psaC_p5	AATCTCGAGTAAGAGGCCGA	GCTATTGGACCCGCTATTGT	50.0	50.0	57.9	57.7	1023 bp	1.0	78.6