Hotspot

petD

LSC · 77586-78825 · 1240 bp

Pi 0.0078

MarkerSeek score 39.80

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1240
variable_sites: 15
pi: 0.0078
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TGGTTATTGGGCGGTGAAAA	GAAGCCTCCCGGAATTTGAT	45.0	50.0	58.0	57.9	2159-2160 bp	1.0	80.3
petD_p2	TTGGTTATTGGGCGGTGAAA	GAAGCCTCCCGGAATTTGAT	45.0	50.0	58.0	57.9	2160-2161 bp	1.0	80.3
petD_p3	TATCGTCCGACGGTTACTGA	GAAGCCTCCCGGAATTTGAT	50.0	50.0	58.0	57.9	2416-2417 bp	1.0	80.1
petD_p4	ACTATCGTCCGACGGTTACT	GAAGCCTCCCGGAATTTGAT	50.0	50.0	58.0	57.9	2418-2419 bp	1.0	80.0
petD_p5	GCCGCTTCTTACTGCTGTAT	GAAGCCTCCCGGAATTTGAT	50.0	50.0	58.1	57.9	2015-2016 bp	1.0	79.9