Hotspot

ndhF

SSC · 110350-112566 · 2217 bp

Pi 0.00308

MarkerSeek score 45.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 12
pi: 0.00308
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGATGCAACAACAAGACGTT	AACAAGGGAAAATACGCCGA	45.0	45.0	57.6	57.8	2731-2746 bp	1.0	76.2
ndhF_p2	CGATGCAACAACAAGACGTT	ACAAGGGAAAATACGCCGAA	45.0	45.0	57.6	57.8	2730-2745 bp	1.0	76.2
ndhF_p3	TCGATGCAACAACAAGACGT	AACAAGGGAAAATACGCCGA	45.0	45.0	58.4	57.8	2732-2747 bp	1.0	75.9
ndhF_p4	TCGATGCAACAACAAGACGT	ACAAGGGAAAATACGCCGAA	45.0	45.0	58.4	57.8	2731-2746 bp	1.0	75.9
ndhF_p5	TTCGATGCAACAACAAGACG	ACAAGGGAAAATACGCCGAA	45.0	45.0	57.3	57.8	2732-2747 bp	1.0	74.0