Hotspot

psbT-psbN

LSC · 75394-75458 · 65 bp

Back to Anna

Pi 0.041
MarkerSeek score 56.80
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
65
variable_sites
4
pi
0.041
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 TCGAGAACCGCCTAAAGTTC TCGAGGAACATGGGGACTAA 50.0 50.0 57.6 57.8 119 bp 1.0 77.9
psbT-psbN_p2 TCGAGAACCGCCTAAAGTTC TTCGAGGAACATGGGGACTA 50.0 50.0 57.6 57.8 120 bp 1.0 77.9
psbT-psbN_p3 TCGAGAACCGCCTAAAGTTC CATTCGAGGAACATGGGGAC 50.0 55.0 57.6 58.3 122 bp 1.0 77.1
psbT-psbN_p4 GAGAACCGCCTAAAGTTCCA TCGAGGAACATGGGGACTAA 50.0 50.0 57.5 57.8 117 bp 1.0 77.1
psbT-psbN_p5 GAGAACCGCCTAAAGTTCCA TTCGAGGAACATGGGGACTA 50.0 50.0 57.5 57.8 118 bp 1.0 77.0