Hotspot

psaI-ycf4

LSC · 60615-61044 · 430 bp

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Pi 0.00349
MarkerSeek score 57.90
Primer available yes
Label psaI-ycf4

Feature Metadata

label_name
psaI-ycf4
feature_type
igs
region
LSC
length_bp
430
variable_sites
3
pi
0.00349
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-ycf4_p1 ATTTCCGGCAATTGCAATGG AGGGCCCAGCAAAAATTACT 45.0 45.0 58.0 57.7 567-568 bp 1.0 77.7
psaI-ycf4_p2 TAGGCCTAGTATTTCCGGCA AGGGCCCAGCAAAAATTACT 50.0 45.0 57.9 57.7 577-578 bp 1.0 77.2
psaI-ycf4_p3 GGCAATTGCAATGGCTTCTT AGGGCCCAGCAAAAATTACT 45.0 45.0 57.9 57.7 561-562 bp 1.0 77.1
psaI-ycf4_p4 GGCCTAGTATTTCCGGCAAT AGGGCCCAGCAAAAATTACT 50.0 45.0 57.7 57.7 575-576 bp 1.0 75.8
psaI-ycf4_p5 ATTTCCGGCAATTGCAATGG CTTAGTTTTCGAGACCCCGT 45.0 50.0 58.0 57.3 549-550 bp 1.0 74.5