Hotspot

rps2-rpoC2

LSC · 16791-16993 · 203 bp

Back to Anna

Pi 0.00921
MarkerSeek score 56.60
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
203
variable_sites
3
pi
0.00921
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 CCAGCATCTCTTCCAAGGTG TTCGAGGGGGAAATGAGAGA 55.0 50.0 58.3 57.8 356-363 bp 1.0 78.5
rps2-rpoC2_p2 CCTCCAGCATCTCTTCCAAG TTCGAGGGGGAAATGAGAGA 55.0 50.0 57.7 57.8 359-366 bp 1.0 77.8
rps2-rpoC2_p3 CTCCAGCATCTCTTCCAAGG TTCGAGGGGGAAATGAGAGA 55.0 50.0 57.7 57.8 358-365 bp 1.0 77.8
rps2-rpoC2_p4 TCCAGCATCTCTTCCAAGGT TTCGAGGGGGAAATGAGAGA 50.0 50.0 58.3 57.8 357-364 bp 1.0 77.8
rps2-rpoC2_p5 CCAGCATCTCTTCCAAGGTG TCGAGGGGGAAATGAGAGAT 55.0 50.0 58.3 57.5 355-362 bp 1.0 76.6