Hotspot

atpH-atpI

LSC · 14056-15110 · 1055 bp

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Pi 0.00458
MarkerSeek score 60.80
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1055
variable_sites
9
pi
0.00458
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 TCCGATAGAAGCAAGCCCTA AGTGGTTCCCATACCTGTCA 50.0 50.0 57.9 58.0 1228-1239 bp 1.0 80.4
atpH-atpI_p2 TCCGATAGAAGCAAGCCCTA TCAGTGGTTCCCATACCTGT 50.0 50.0 57.9 58.0 1230-1241 bp 1.0 80.4
atpH-atpI_p3 AACGGAAGCAGCAGAAATCA AGTGGTTCCCATACCTGTCA 45.0 50.0 58.1 58.0 1189-1200 bp 1.0 80.3
atpH-atpI_p4 AACGGAAGCAGCAGAAATCA TCAGTGGTTCCCATACCTGT 45.0 50.0 58.1 58.0 1191-1202 bp 1.0 80.3
atpH-atpI_p5 TCCAGGTCCGATAGAAGCAA AGTGGTTCCCATACCTGTCA 50.0 50.0 58.1 58.0 1234-1245 bp 1.0 79.9