Hotspot

psbK-psbI

LSC · 7920-8342 · 423 bp

Back to Anna

Pi 0.00473
MarkerSeek score 57.90
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
423
variable_sites
4
pi
0.00473
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 CTTTTGTTTGGCAAGCTGCT GGATTACGTCCCGGATCATT 45.0 50.0 58.1 57.2 552-553 bp 1.0 73.6
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG GGATTACGTCCCGGATCATT 50.0 50.0 57.9 57.2 547-548 bp 1.0 73.6
psbK-psbI_p3 CTTTTGTTTGGCAAGCTGCT TCTTCACGTCCAGGATTACG 45.0 50.0 58.1 57.1 564-565 bp 1.0 72.8
psbK-psbI_p4 GTTTGGCAAGCTGCTGTAAG TCTTCACGTCCAGGATTACG 50.0 50.0 57.9 57.1 559-560 bp 1.0 72.8
psbK-psbI_p5 TGGCAAGCTGCTGTAAGTTT GGATTACGTCCCGGATCATT 45.0 50.0 58.3 57.2 544-545 bp 1.0 71.6