Hotspot

ycf1

SSC · 125948-131641 · 5694 bp

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Pi 0.00353
MarkerSeek score 54.90
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5694
variable_sites
20
pi
0.00353
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 ACAGATGTCGTTTCTAGTCT GGCGGACGCATATCTTCTTA 40.0 50.0 53.5 57.6 1675-1679 bp 1.0 41.0
ycf1_p2 AACAGATGTCGTTTCTAGTCT GGCGGACGCATATCTTCTTA 38.1 50.0 54.4 57.6 1676-1680 bp 1.0 40.7
ycf1_p3 ACAGATGTCGTTTCTAGTCT GGCGGACGCATATCTTCTTAT 40.0 47.6 53.5 58.0 1675-1679 bp 1.0 40.7
ycf1_p4 ACATAACAGATGTCGTTTCTAGTCT GGCGGACGCATATCTTCTTA 36.0 50.0 57.8 57.6 1680-1684 bp 1.0 40.7
ycf1_p5 ACAGATGTCGTTTCTAGTCT CAAATATATAGGGGGCGGACG 40.0 52.4 53.5 58.1 1688-1692 bp 1.0 40.7
ycf1_p1 AGACGTTCTGTTATATTGAAGAACA ACACAACTCTTTCTTGAATCAACA 32.0 33.3 56.6 57.2 267 bp 1.0 41.2
ycf1_p2 AGACGTTCTGTTATATTGAAGAACAG ACACAACTCTTTCTTGAATCAACA 34.6 33.3 57.5 57.2 267 bp 1.0 41.2
ycf1_p3 GACGTTCTGTTATATTGAAGAACAG ACACAACTCTTTCTTGAATCAACA 36.0 33.3 56.3 57.2 266 bp 1.0 41.2
ycf1_p4 GACGTTCTGTTATATTGAAGAACA ACACAACTCTTTCTTGAATCAACA 33.3 33.3 55.3 57.2 266 bp 1.0 41.2
ycf1_p5 ACGTTCTGTTATATTGAAGAACAG ACACAACTCTTTCTTGAATCAACA 33.3 33.3 55.3 57.2 265 bp 1.0 41.2