Hotspot

trnN-GUU-trnR-ACG

IRa · 132043-132622 · 580 bp

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Pi 0.00862

MarkerSeek score 63.30

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 580
variable_sites: 5
pi: 0.00862
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	673 bp	1.0	77.6
trnN-GUU-trnR-ACG_p2	TGGGAACGGGAATTGAACTC	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	758 bp	1.0	77.2
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	GAACAGATCCAGTGGAGACG	50.0	55.0	58.2	57.7	710 bp	1.0	77.0
trnN-GUU-trnR-ACG_p4	TGGGAACGGGAATTGAACTC	GAACAGATCCAGTGGAGACG	50.0	55.0	57.8	57.7	795 bp	1.0	76.7
trnN-GUU-trnR-ACG_p5	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	677 bp	1.0	76.7