Hotspot

ndhF-rpl32

SSC · 114664-115432 · 769 bp

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Pi 0.0091
MarkerSeek score 58.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
769
variable_sites
7
pi
0.0091
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CGGCGAAAACTTTTTGTTGC TGCTACCCAATATCCCTTCCT 45.0 47.6 57.7 57.9 966-967 bp 1.0 69.2
ndhF-rpl32_p2 GCGAAAACTTTTTGTTGCCG TGCTACCCAATATCCCTTCCT 45.0 47.6 57.7 57.9 964-965 bp 1.0 69.2
ndhF-rpl32_p3 GCGAAAACTTTTTGTTGCCG TGATGTCGAAGTGCGTTTTT 45.0 40.0 57.7 56.6 913-914 bp 1.0 66.6
ndhF-rpl32_p4 GCGAAAACTTTTTGTTGCCG TTGATGTCGAAGTGCGTTTT 45.0 40.0 57.7 56.6 914-915 bp 1.0 66.6
ndhF-rpl32_p5 GCGAAAACTTTTTGTTGCCG TTTGATGTCGAAGTGCGTTT 45.0 40.0 57.7 56.6 915-916 bp 1.0 66.6