Hotspot

trnR-ACG-trnN-GUU

IRb · 109990-110570 · 581 bp

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Pi 0.00861

MarkerSeek score 63.30

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 581
variable_sites: 5
pi: 0.00861
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	673 bp	1.0	77.6
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	TGGGAACGGGAATTGAACTC	55.0	50.0	57.8	57.8	758 bp	1.0	77.2
trnR-ACG-trnN-GUU_p3	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.7	58.2	710 bp	1.0	77.0
trnR-ACG-trnN-GUU_p4	GAACAGATCCAGTGGAGACG	TGGGAACGGGAATTGAACTC	55.0	50.0	57.7	57.8	795 bp	1.0	76.7
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	CGATTGGTCGTAGGTTCGAA	55.0	50.0	57.8	57.7	677 bp	1.0	76.7