Hotspot

rrn5-trnR-ACG

IRb · 109655-109915 · 261 bp

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Pi 0

MarkerSeek score 33.80

Primer available yes

Label rrn5-trnR-ACG

Feature Metadata

label_name: rrn5-trnR-ACG
feature_type: igs
region: IRb
length_bp: 261
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnR-ACG_p1	GGTGACGATACTGTAGGGGA	CCACGTGCTCTAATCCTCTG	55.0	55.0	57.9	57.8	345 bp	1.0	77.8
rrn5-trnR-ACG_p2	AATCCATCCCGAACTTGGTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	380 bp	1.0	77.2
rrn5-trnR-ACG_p3	ACCAATCCATCCCGAACTTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	383 bp	1.0	77.2
rrn5-trnR-ACG_p4	CAATCCATCCCGAACTTGGT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	381 bp	1.0	77.2
rrn5-trnR-ACG_p5	CACCAATCCATCCCGAACTT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	384 bp	1.0	77.2