Hotspot

psaA-ycf3

LSC · 43057-43827 · 771 bp

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Pi 0.00395

MarkerSeek score 62.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 3
pi: 0.00395
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	942-948 bp	1.0	80.1
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	924-930 bp	1.0	80.1
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	938-944 bp	1.0	78.3
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	940-946 bp	1.0	78.3
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCCGGTAAT	50.0	50.0	57.9	58.3	912-918 bp	1.0	78.0