Hotspot

psbM-trnD-GUC

LSC · 30512-31590 · 1079 bp

Back to Anisodus

Pi 0.00556
MarkerSeek score 61.90
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1079
variable_sites
6
pi
0.00556
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGTAGAAAAGCGGTAGGAACT CCCGTATTTCCCTGGGATTG 42.9 55.0 56.4 58.0 1234-1237 bp 1.0 59.3
psbM-trnD-GUC_p2 AGTAGAAAAGCGGTAGGAACT TCCCGTATTTCCCTGGGATT 42.9 50.0 56.4 58.1 1235-1238 bp 1.0 58.7
psbM-trnD-GUC_p3 AAGTAGAAAAGCGGTAGGAACT CCCGTATTTCCCTGGGATTG 40.9 55.0 57.1 58.0 1235-1238 bp 1.0 57.1
psbM-trnD-GUC_p4 AGAAAAGCGGTAGGAACTAGA CCCGTATTTCCCTGGGATTG 42.9 55.0 56.1 58.0 1231-1234 bp 1.0 57.1
psbM-trnD-GUC_p5 AAGTAGAAAAGCGGTAGGAACT TCCCGTATTTCCCTGGGATT 40.9 50.0 57.1 58.1 1236-1239 bp 1.0 56.5