Hotspot

rpoB-trnC-GCA

LSC · 27268-28562 · 1295 bp

Back to Anisodus

Pi 0.00772

MarkerSeek score 63.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1295
variable_sites: 10
pi: 0.00772
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTCATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	57.9	50.0	58.0	58.1	1379 bp	1.0	71.8
rpoB-trnC-GCA_p2	ATTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1384 bp	1.0	71.3
rpoB-trnC-GCA_p3	TCATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.2	58.1	1378 bp	1.0	70.7
rpoB-trnC-GCA_p4	CTCATTTCCATCCCCGAGC	AGTCCTCTGCCTTACCACTC	57.9	55.0	58.0	58.4	1348 bp	1.0	69.0
rpoB-trnC-GCA_p5	CATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	52.6	50.0	57.6	58.1	1377 bp	1.0	68.5