Hotspot

ycf1

SSC · 119911-125427 · 5517 bp

Back to Angraecum

Pi 0.0159

MarkerSeek score 48.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5517
variable_sites: 130
pi: 0.0159
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTATCCAAATTTGTATTCCTATCA	TGAATCAAAAAGACAAATGGAA	28.0	27.3	53.8	52.7	227-248 bp	1.0	43.0
ycf1_p2	TCTATCCAAATTTGTATTCCTATCA	TGAATCAAAAAGACAAATGGAAA	28.0	26.1	53.8	53.6	227-248 bp	1.0	43.0
ycf1_p3	TTTTCTATCCAAATTTGTATTCCT	TGAATCAAAAAGACAAATGGAA	25.0	27.3	52.6	52.7	230-251 bp	1.0	43.0
ycf1_p4	TCTATCCAAATTTGTATTCCTATCA	ATGAATCAAAAAGACAAATGGA	28.0	27.3	53.8	52.5	228-249 bp	1.0	43.0
ycf1_p5	TCTATCCAAATTTGTATTCCTATCAA	TGAATCAAAAAGACAAATGGAA	26.9	27.3	54.5	52.7	227-248 bp	1.0	43.0