Hotspot

ndhD-psaC

SSC · 117170-117668 · 499 bp

Back to Angraecum

Pi 0.0275

MarkerSeek score 64.50

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 499
variable_sites: 19
pi: 0.0275
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TTTCTCCTCCCCACATGGAT	GCTTGTCCCACGGATGTATT	50.0	50.0	58.0	58.0	746-801 bp	1.0	86.0
ndhD-psaC_p2	TTTCTCCTCCCCACATGGAT	GTACCCAATGTGTTCGAGCT	50.0	50.0	58.0	57.9	763-818 bp	1.0	85.5
ndhD-psaC_p3	ACTCATTCCTGGCAATGCAA	GCTTGTCCCACGGATGTATT	45.0	50.0	58.1	58.0	1418-1426 bp	0.666667	71.0
ndhD-psaC_p4	TTTCTCCTCCCCACATGGAT	AAGATGTGAATCCGCCTGTC	50.0	50.0	58.0	58.0	648 bp	0.333333	53.0
ndhD-psaC_p5	ACTCATTCCTGGCAATGCAA	AAGATGTGAATCCGCCTGTC	45.0	50.0	58.1	58.0	1320 bp	0.333333	52.5