Hotspot

clpP-psbB

LSC · 74522-75404 · 883 bp

Back to Angraecum

Pi 0.0313

MarkerSeek score 78.10

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 883
variable_sites: 38
pi: 0.0313
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	1043-1072 bp	1.0	85.9
clpP-psbB_p2	TATACGTCAACCCAAGCTGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	1038-1067 bp	1.0	85.8
clpP-psbB_p3	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	961-990 bp	1.0	85.2
clpP-psbB_p4	TATACGTCAACCCAAGCTGC	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	956-985 bp	1.0	85.1
clpP-psbB_p5	CTTTTGGAACACCAATGGGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.8	57.9	989-1018 bp	1.0	84.8