Hotspot

rbcL-accD

LSC · 57862-58673 · 812 bp

Back to Angraecum

Pi 0.019

MarkerSeek score 68.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 812
variable_sites: 23
pi: 0.019
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCCGCTGCTTGTGAAATATG	CCACTGGTATGTCCAAGAGC	50.0	55.0	58.2	58.0	997-999 bp	1.0	80.7
rbcL-accD_p2	GTTCGACCCAGTGGATAAGC	CCACTGGTATGTCCAAGAGC	55.0	55.0	58.3	58.0	959-961 bp	1.0	79.4
rbcL-accD_p3	TTCGACCCAGTGGATAAGCT	CCACTGGTATGTCCAAGAGC	50.0	55.0	58.4	58.0	958-960 bp	1.0	78.7
rbcL-accD_p4	TCGACCCAGTGGATAAGCTA	CCACTGGTATGTCCAAGAGC	50.0	55.0	57.6	58.0	957-959 bp	1.0	78.6
rbcL-accD_p5	CCGCTGCTTGTGAAATATGG	CCACTGGTATGTCCAAGAGC	50.0	55.0	57.5	58.0	996-998 bp	1.0	78.2