Hotspot

atpB-rbcL

LSC · 55383-56397 · 1015 bp

Back to Angraecum

Pi 0.0204

MarkerSeek score 68.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1015
variable_sites: 31
pi: 0.0204
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATCTTGCCCGGTGGAAAAAT	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.1	58.1	1163-1176 bp	1.0	80.9
atpB-rbcL_p2	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	1131-1144 bp	1.0	80.3
atpB-rbcL_p3	CGATACGCCCTAGGTTTTGT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.7	58.1	1114-1127 bp	1.0	79.0
atpB-rbcL_p4	ATCTTGCCCGGTGGAAAAAT	TGGTGACATAAGTCCCTCCC	45.0	55.0	58.1	58.4	1164-1177 bp	1.0	78.5
atpB-rbcL_p5	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	45.0	55.0	57.9	58.4	1132-1145 bp	1.0	77.9